Table 1.

PCR primers used in the RT-PCR protocol for astrocytes and pyramidal cells

Gene accession #	First PCR primers	Size (bp)	Second PCR nested primers	Size (bp)
GFAP	Sense, 454 AGCTCCAAGATGAAACCAACCT^{^a}	631	Sense, 509 ACAGACAGGAGGCGGATGAA	263
NM_017009	Antisense, 1066 CGATCTCGATGTCCAGGGC^{^a}		Antisense, 749 GGACCGATACCACTCTTCTGTTT
S100β	Sense, 45 CATCAGTATTCAGGGAGAGAGGG	166	Sense, 58 GGGAGAGAGGGTGACAAGCA	132
NM_013191.1	Antisense, 191 GTCACACTCCCCATCCCCAT		Antisense, 170 TTCGTCCAGCGTCTCCATCA
COX-1	Sense, 27 GTTTCCCCTGCTGCTGCTC	359	Sense, 137 AGGGTGTCTGTGTCCGCTTC	185
NM_017043.1	Antisense, 366 GGCTGGGGATAAGGTTGGAC		Antisense, 301 GGCATTCACAAACTCCCAGAT
COX-2	Sense, 199 CTGAAGCCCACCCCAAACAC	247	Sense, 202 AAGCCCACCCCAAACACAGT	206
NM_017232.2	Antisense 426 TGGGAGTTGGGCAGTCATCA		Antisense, 384 CTGGTGTAGTAGGAGAGGTTGGAG
VGluT1	^{^b}Sense, 361 GGCTCCTTTTTCTGGGGGTAC^{^b}	259	Sense, 373 TGGGGGTACATTGTCACTCAGA^{^b}	201
NM_053859.1	Antisense, 600 CCAGCCGACTCCGTTCTAAG^{^b}		Antisense, 553 ATGGCAAGCAGGGTATGTGAC^{^b}