Table 2.

Relative intensity of hybridization in thalamic nuclei

α1α2α3α4α5β1β2β3γ1γ2
AV0.450.470.510.480.760.260.680.600.220.23
AM0.340.470.460.460.670.260.560.640.230.23
AD0.360.240.620.290.510.400.450.640.220.47
VA0.230.360.290.240.170.190.270.570.210.22
Vla0.230.280.400.400.140.200.160.540.210.34
Vlp0.380.320.390.380.290.240.220.470.190.21
VPL0.390.450.370.550.420.260.450.470.290.43
VPM0.480.510.430.650.480.280.470.540.360.49
VPI0.200.280.320.570.380.170.240.370.210.11
VMb0.260.670.640.830.570.310.450.590.280.45
MDm0.200.520.710.590.430.250.360.550.360.31
MDl0.210.490.680.640.480.270.390.410.300.37
Rh0.210.680.700.470.540.470.350.850.400.25
CeM0.240.640.790.490.350.770.480.590.450.31
Pc0.150.520.530.360.180.380.170.430.240.22
CM0.220.440.290.280.140.420.320.580.360.30
CL0.270.650.660.310.100.360.330.720.370.41
Pf0.401.01.00.380.221.00.620.781.00.71
LD0.410.420.570.410.520.370.430.410.370.32
LP0.870.930.640.900.810.670.830.780.550.79
LGmc1.00.500.610.850.690.431.00.650.311.0
LGpc0.910.680.280.840.590.540.950.640.430.97
MGad0.340.680.680.770.650.230.350.510.290.36
MGv0.410.510.550.560.690.220.460.450.170.47
Pla0.200.310.370.520.340.210.260.470.270.26
Pli0.810.870.570.981.00.540.510.840.320.69
Pll0.520.620.310.590.520.400.330.820.220.41
Plm0.770.850.411.00.770.560.481.00.390.63
Po0.270.330.220.370.390.270.180.650.250.22
SGL0.720.820.890.540.600.630.370.820.570.55
R0.010.100.180.050.040.070.010.140.080.11
  • Optical density readings were obtained from film autoradiograms in individual nuclei. Values from multiple in situhybridization runs were averaged for all cRNA probes, and individual values were compared against each other for the estimation of the intensity of cRNA probe labeling in individual nuclei. The optical density value for each nucleus was divided by the value found in the nucleus with the highest density value for each probe. The density in the nucleus with the highest intensity for each cRNA probe is thus 1.0, and the other values are fractions of this value. Hybridization levels of 33P-labeled cRNA probes were converted from density units to units of radioactivity using 14C standards exposed on the same sheet of film (see Materials and Methods).