Number of DRG neurons after antibody treatment
| Nonimmune Fab | Anti-trkC Fab | Reduction (%) | ||
|---|---|---|---|---|
| St.31 (E7–7.5) | ||||
| Total | 8538 ± 916 (n = 3) | 4486 ± 543 (n = 4) | 47 | p ≤ 0.005 |
| VL | 2246 ± 727 | 516 ± 84 | 77 | p ≤ 0.02 |
| DM | 6292 ± 289 | 3970 ± 564 | 36 | p ≤ 0.01 |
| St.26 (E5) | ||||
| Total | 4045 ± 254 (n = 4) | 2933 ± 365 (n = 4) | 27 | p ≤ 0.02 |
For the older embryos (E7/E7.5) all neurons were counted in every sixth section of brachial ganglion 14. VL- and DM-located neurons could be distinguished at this age on the basis of location and diameter (see Materials and Methods). Significance levels were determined by the one-tailed Student’s t test.