Control | 0 Ca2+ | 0 Cl− | 0 Na+ | DIDS | |
---|---|---|---|---|---|
n = 289 | n = 41 | n = 31 | n = 44 | n = 42 | |
[H+]i(nm) | 54.6 ± 14.9 | 50.5 ± 5.7 | **30.3 ± 5.2 | **171.1 ± 56.6 | **108.5 ± 20.3 |
Alkaline (%) | 100 | 96 ± 34 | **52 ± 31 | **18 ± 29 | **25 ± 29 |
Acid (%) | 100 | 102 ± 27 | *129 ± 51 | **0 | **0 |
Baseline values of [H+]i (in nm) and [H+]i changes (shown as % of control) in CO2/HCO3−-buffered saline (control), after removal of extracellular Ca2+ (0 Ca2+), Cl− (0 Cl−), or Na+ (0 Na+), or after addition of the anion transport blocker 4,4′-diisothiocyanato-stilbene-2,2′-disulphonic acid (DIDS) (0.5 mm) are shown. Percentage values in the second and third rows indicate relative amplitudes of kainate-induced alkaline and acid shifts in comparison to the control. Shown are n = number of cells, and means ± SD. Asterisks indicate significant differences as compared with control values in CO2/HCO3−-buffered saline; **p ≤ 0.001; *p ≤ 0.01.