Immunocytochemical characterization of the cultured utricular epithelial cells
| Markers | Immunopositivity |
|---|---|
| General epithelial cell antigens | |
| ZO1 (Tight junction protein) | + |
| F-actin | + |
| Cytokeratin | + |
| Fibroblast antigens | |
| Vimentin | − |
| Thy 1.1 | − |
| Glial cell antigens | |
| GFAP | − |
| Myelin (oligodendrocyte antigen) | − |
| Neuronal antigen | |
| NF | − |
Utricular epithelial cells were prepared from P4–P5 rats and plated in poly-d-lysine-coated 16-well Lab-Tek culture slides in 5% FBS-supplemented medium for 48 hr. The cultures were fixed with 4% paraformaldehyde and then were stained with a phalloidin-FITC conjugate or the antibodies listed above.