Antiserum | Host species | Dilution | Source | Demonstration of specificity, citations |
---|---|---|---|---|
Glutamate | Rabbit | 1:500 | Chemicon International Inc. (Temecula, CA) | Marc et al., 1990; Kalloniatis and Fletcher, 1993 |
GluR1 | Rabbit | 1.0 μg/ml | Gift of Dr. R. J. Wenthold, NIH1-a | Petralia and Wenthold, 1992; Wenthold et al., 1992 |
GluR2/3 | Rabbit | 0.5 μg/ml | Gift of Dr. R. J. Wenthold, NIH | Petralia and Wenthold, 1992; Wenthold et al., 1992; recognizes both subunit proteins |
GluR4 | Rabbit | 1.0 μg/ml | Gift of Dr. R. J. Wenthold, NIH | Petralia and Wenthold, 1992; Wenthold et al., 1992 |
NR1 | Rabbit | 1.0 μg/ml | Gift of Dr. R. J. Wenthold, NIH | Petralia et al., 1994b; directed against the C terminus of the rat NMDAR 1 receptor and recognizes 4 of the 8 receptor splice variants |
NR2A/B | Rabbit | 1.0 μg/ml | Gift of Dr. R. J. Wenthold, NIH | Petralia et al., 1994a; recognizes the A and B subunits of the NMDAR2 receptor |
EAAC1 | Rabbit | 0.06 μg/ml | Laboratory of J. Rothstein | Rothstein et al., 1993 |
Substance P | Rat | 1:1000 | Accurate Chemical Co. (Westbury, NY) | Cuello et al., 1979; Mawe and Gershon 1989; this antibody is directed against the 5-8 C-terminal fragment and thus will not react with substance K (neuromedin A) |
ChAT | Goat | 1:300 | Chemicon International Inc. | Neunlist and Schemann, 1997 |
Nitric oxide synthase | Mouse | 1:1000 | Transduction Labs (Lexington, KY) | This antibody is directed against amino acids 1095-1289 of human brain (NOS) |
Calretinin | Goat | 1:6000 | Chemicon International Inc. | Jacobowitz and Winsky, 1991 |
Calbindin | Mouse | 1:100 | Sigma | Reacts specifically with calbindin-D (28 kDa) |
Vasoactive intestinal polypeptide | Mouse | 1:500 | CURE/Gastroenteric Biology Center, antibody/RIA core, NIH Grant DK41301 | McConalogue et al., 1995; Wong et al., 1996 |
↵F1-a NIH, National Institutes of Health.