The nucleotide positions of primers and protocols of PCR reactions
| Names of channels | Positions of primers | Base pairs of bands | Protocols of PCR |
|---|---|---|---|
| ROMK1 (Kir1.1) | 67 ∼ 84, 522 ∼ 538 | 472 | 94°C, 45 sec; 60°C, 1 min 30 sec; 72°C, 2 min |
| *793 ∼ 812, 1100 ∼ 1119 | 327 | 94°C, 1 min; 68°C, 1 min; 72°C, 2 min | |
| IRK1 (Kir2.1) | 1232 ∼ 1249, 1517 ∼ 1534 | 303 | 94°C, 1 min; 58°C, 1 min 15 sec; 72°C, 2 min |
| IRK2 (Kir2.2) | 1288 ∼ 1305, 1523 ∼ 1539 | 252 | 94°C, 1 min; 68°C, 1 min; 72°C, 1 min 30 sec |
| *152 ∼ 172, 847 ∼ 866 | 715 | 94°C, 1 min; 68°C, 1 min; 72°C, 2 min | |
| IRK3 (Kir2.3) | 304 ∼ 323, 1198 ∼ 1223 | 820 | 94°C, 1 min; 68°C, 1 min; 72°C, 1 min |
| GIRK1 (Kir3.1) | 1192 ∼ 1209, 1492 ∼ 1509 | 318 | 94°C, 1 min; 57°C, 1 min 30 sec; 72°C, 2 min |
| GIRK2 (Kir3.2) | 914 ∼ 933, 1259 ∼ 1277 | 364 | 94°C, 1 min; 64°C, 1 min; 72°C, 2 min |
| GIRK3 (Kir3.3) | 611 ∼ 630, 1048 ∼ 1067 | 457 | 94°C, 1 min; 64°C, 1 min; 72°C, 2 min |
| GIRK4 (Kir3.4) | 904 ∼ 923, 1246 ∼ 1265 | 362 | 94°C, 1 min; 68°C, 1 min; 72°C, 1 min |
| *4 ∼ 22, 362 ∼ 380 | 377 | 94°C, 1 min; 63°C, 1 min 30 sec; 72°C, 1 min 40 sec | |
| KAB-2/BIR10 (Kir4.1) | 312 ∼ 329, 966 ∼ 983 | 672 | 94°C, 45 sec; 60°C, 1 min; 72°C, 2 min |
| uKATP-1 (Kir6.1) | 566 ∼ 583, 943 ∼ 959 | 394 | 94°C, 1 min; 68°C, 1 min; 72°C, 1 min 30 sec |
| *401 ∼ 418, 704 ∼ 722 | 322 | 94°C, 1 min; 63°C, 1 min 30 sec; 72°C, 1 min 40 sec | |
| BIR (Kir6.2) | 198 ∼ 215, 623 ∼ 642 | 445 | 94°C, 1 min; 60°C, 1 min; 72°C, 1 min |
The sets of primers indicated by asterisks were also used, but the data were not shown in Figure 2.