Effects of PE, CPA, and PDC on glutamate uptake in astrocytes from tegmentum
| PE (100 μm) | CPA (100 nm) | PE + CPA | PDC (1 mm) |
|---|---|---|---|
| 98 ± 6 | 94 ± 8 | 90 ± 4 | 18 ± 4 |
Glutamate uptake was determined in incubation buffer supplemented with 10 mm LiCl in the presence or absence of PDC. Reaction was initiated by addition of a glutamate solution containing [3H]glutamate and stopped after 20 min (for details, see Material and Methods). Data are percentages of glutamate uptake from unstimulated cells (4230 ± 370 cpm = 100%) and given as mean ± SD from three independent experiments.