Table 1.

Tabulation of nodal intermediates stained by ankyrinG at different times during myelination of the rat sciatic nerve

Postnatal dayNumber of nodes observedRelative % of single clustersRelative % of paired clusters (5–10 μm)Relative % of paired clusters (<5 μm)Relative % of mature nodes
  • Cryosections (4 μm) were prepared from the same areas of three separate sciatic nerves, at each time point, and double-stained for ankyrinG and MAG. No significant variation in staining was noted between different nerves from the same time point. Single clusters were distinguished from mature nodes by the absence of MAG staining on one side of the cluster.