Table 1.

Kinetics of mIPSCs and outside-out patch responses induced by GABA in neocortical pyramidal neurons

	Rise time (20–80%)	τ_fast (msec)	% Fast	τ_slow (msec)	n
mIPSCs	0.46 ± 0.05	7.5 ± 1	76.3 ± 10	33.2 ± 11	25
Patch currents
10 μm (N)	0.54 ± 0.15	4.5 ± 2	66.2 ± 8	34.4 ± 12	4
50 μm	0.73 ± 0.09	3.6 ± 2	57.0 ± 8	60.2 ± 12	7
100 μm	0.55 ± 0.04	4.4 ± 2	47.1 ± 7	70.1 ± 20	12
300 μm	0.52 ± 0.03	5.2 ± 3	46.5 ± 16	68.8 ± 27	8
1 mm	0.37 ± 0.03	5.1 ± 2	49.4 ± 10	62.4 ± 14	21
1 mm (N)	0.50 ± 0.07	10.2 ± 1	55.7 ± 9	56.1 ± 13	6
10 mm	0.30 ± 0.04	6.0 ± 3	52.0 ± 8	58.8 ± 17	16

Outside-out patch responses were induced by rapid application of GABA pulses of 1 msec duration. Both mIPSCs and patch responses were recorded at a holding potential of −70 mV. Currents were fit with the sum of two exponential functions, as explained in Materials and Methods. The slow component of patch responses was significantly slower and larger than that of mIPSCs at all of the tested concentrations except 10 μm (p < 0.05). In addition, the fast time constants of patch responses and mIPSCs were found significantly different at 10, 50, and 100 μm and 1 mm concentrations. Data are shown as mean ± SD. (N), Nucleated outside-out patches; n, number of experiments.