DHR123 oxidation to RH123 during hypoxia–hypoglycemia and reoxygenation in CA1 cells
| n | ↑ F | ↓ F | Stable | CNQX/AP-5 | ||||
|---|---|---|---|---|---|---|---|---|
| n | ↑ F | ↓ F | Stable | |||||
| H/H 2–4 min | 200 | 27% | 0% | 73% | 210 | 0.9% | 30.9% | 68.1% |
| H/H 4–8 min | 183 | 30.6% | 66.7% | 2.7% | 209 | 0.4% | 94.7% | 4.8% |
| Reoxygenation | 208 | 81.7% | 3.4% | 14.9% | 200 | 21.5% | 45.5% | 33% |
RH123 fluorescence was measured in individual pyramidal cells of the CA1 layer during a hypoxic–hypoglycemic (H/H) episode of 8 min and reoxygenation. ↑ F and ↓ F indicate increase or decrease in fluorescence, respectively. First and fifth columns (n) indicate the number of cells. Most cells had a stable level of fluorescence emission during the first half of the anoxic insult (first row), and either decreased or increased during the second half (second row). Fluorescence increased in the majority of cells during reoxygenation (third row). Glutamate receptor blockers (see text) in general caused a decrease in DHR123 oxidation at all stages (columns 5–8), as shown by the low percentage of cells whose fluorescence increased (sixth column).