Sublethal oxygen–glucose deprivation (OGD) increases kainate-mediated excitotoxicity
| Condition | Cell death (%) | p | |
|---|---|---|---|
| Sham wash | OGD | ||
| Blank | 0 ± 1.8 | ||
| 30 μm kainate | 38.4 ± 2.13-a | 58.8 ± 4.4a,b | 0.0002 |
| 150 μmNMDA | 44.5 ± 4.13-a | 36.4 ± 3.13-a | 0.114 |
| 100 min OGD | 59.5 ± 3.43-a | 44.7 ± 1.43-a | 0.007 |
Kainate or NMDA was applied at the indicated concentrations for 24 hr to cultures subjected to a 30 min sublethal oxygen–glucose deprivation or sham wash 24 hr before excitotoxin exposure. Toxin-specific cell death was estimated by measuring LDH efflux into the bathing medium at the end of the excitotoxin exposure, subtracting LDH efflux from sham-washed cultures, and scaling to the mean LDH value corresponding to near-complete neuronal death (100%, induced by 24 hr exposure to 500 μm NMDA) in sister cultures. Sublethal oxygen–glucose deprivation does not affect LDH release induced by 24 hr exposure to 500 μm NMDA. n = 12 cultures per condition, mean ± SEM.
↵F3-a Difference from cell death in cultures exposed to media alone (Blank); p < 0.05 using one-way ANOVA with Bonferroni correction.
F3-b Difference from cell death in corresponding sham wash-conditioned cultures; p = 0.0002, using one-way ANOVA.