Subcellular distribution of rat brainN-acyltransferase activity
| Fraction | Substrate | |||
|---|---|---|---|---|
| di[14C] (16:0) | di[14C] (20:4) | 2-[14C] (16:0) | 2-[14C] (20:4) | |
| Homogenate | 10.0 ± 2.8 | 0.5 ± 0.2 | ND | ND |
| Supernatant 1 | 0.8 ± 0.4 | 0.1 ± 0.1 | ND | ND |
| Particulate | 25.3 ± 4.7 | 1.8 ± 0.5 | ND | ND |
| Soluble | 73.8 ± 12.4 | 5.4 ± 2.2 | 0.7 ± 0.4 | 0.1 ± 0.1 |
| Pellet | 4.3 ± 0.9 | 0.5 ± 0.4 | ND | ND |
| Supernatant 2 | 0.3 ± 0.2 | 0.05 ± 0.05 | ND | ND |
| Microsomes | 8.6 ± 0.3 | 0.4 ± 0.2 | ND | ND |
| Soluble | 23.5 ± 4.2 | 1 ± 0.5 | ND | ND |
| Pellet | 0.4 ± 0.5 | 0.2 ± 0.1 | ND | ND |
N-acyltransferase assays were carried out under standard conditions (see Materials and Methods). RadioactiveN-acyl PEs were purified by monodimensional TLC and quantified by liquid scintillation counting. Results represent the mean ± SEM of 3–4 experiments. di[14C] (16:0), 1,2-di[14]palmitoyl PC; di[14C] (20:4), 1,2-di[14C]arachidonoyl PC; 2-[14C] (16:0), 1-stearoyl,2-[14C]palmitoyl PC; 2-[14C] (20:4), 1-stearoyl,2-[14C]arachidonoyl PC; ND, not determined.