Input subunit percentages | Amplified subunit percentages | ||||
---|---|---|---|---|---|
% NR2A | % NR2B | % NR2C | % NR2A | % NR2B | % NR2C |
81.8 | 9.1 | 9.1 | 63.8 ± 2.7 (3) | 16.9 ± 2.2 (3) | 19.3 ± 0.6 (3) |
9.1 | 81.8 | 9.1 | 19.7 ± 0.9 (3) | 59.8 ± 1.4 (3) | 20.5 ± 2.1 (3) |
9.1 | 9.1 | 81.8 | 19.8 ± 0.4 (3) | 16.0 ± 3.0 (3) | 64.2 ± 3.3 (3) |
33.3 | 33.3 | 33.3 | 38.0 ± 0.5 (3) | 31.4 ± 3.3 (3) | 30.7 ± 2.9 (3) |
% NR2A | % NR2C | % NR2D | % NR2A | % NR2C | % NR2D |
81.8 | 9.1 | 9.1 | 76.1 ± 0.4 (2) | 19.1 ± 0.5 (2) | 4.8 ± 0.1 (2) |
9.1 | 81.8 | 9.1 | 4.7 ± 0.3 (3) | 92.3 ± 0.5 (3) | 3.0 ± 0.3 (3) |
9.1 | 9.1 | 81.8 | 9.8 ± 0.9 (2) | 23.3 ± 0.9 (2) | 66.9 ± 1.8 (2) |
At left, input percentages for different combinations of three NR2 subunit templates are shown. At right, subunit percentages detected by dot-blot hybridization after two rounds of PCR amplification are given as mean ± SD for the number of experiments indicated in parentheses.