Table 2.

Ouabain inhibits O-2A cell proliferation

[3H]thymidine incorporation (% over N1, no growth factors)
PDGFbFGF
Control324  ± 30310  ± 15
Ouabain 10260  ± 262-160283  ± 8
Ouabain 10059  ± 8*75  ± 4*
Ouabain 100038  ± 6*50  ± 4*

  • O-2A progenitor cells were purified and cultured as described previously at a density of 3 × 104cells/cm2 in DME-N1 medium, containing 0.5% FBS (Gallo and Armstrong, 1995). After 2 hr, PDGF or bFGF (both 10 ng/ml), ouabain (10–1000 μm), and [3H]thymidine were added to the culture medium. After 22 hr, [3H]thymidine was measured by trichloroacetic acid precipitation and scintillation counting. Averages of 3 experiments in triplicate ± SEM are shown.

  • ↵* p < 0.01;

  • ↵F2-160 p < 0.005, compared with their respective controls (Student’s t test).