Antisera and dilutions used
| Antiserum | Dilution | Source | |
|---|---|---|---|
| Immunohistochemistry | Western blot | ||
| Rabbit anti-mouse IgG (H + L)2-a | 1:400 | 1:5000 | Jackson ImmunoResearch, West Grove, PA |
| Horse anti-mouse IgG (H + L), biotinylated | 1:400 | 1:2000 | Vector Laboratories, Burlingame, CA |
| Horse anti-mouse Ig γ heavy chain, biotinylated | 1:400 | 1:2000 | Vector Laboratories |
| Goat anti-mouse Ig μ heavy chain, biotinylated | 1:400 | 1:2000 | Vector Laboratories |
| Rabbit anti-mouse Ig κ light chain | 1:2000 | 1:20,000 | Advanced Chemtech, Louisville, KY |
| Rabbit anti-synapsin I | 1:500 | 1:5000 | Dr. John Bixby, University of Miami, Miami, FL |
↵F2-a Recognizes both heavy (H) and light (L) chains of IgG.