Levels of pPVN and mPVN RNA after intraperitoneal and intragastric injection of alcohol
| Time (min) after injection | ||||||
|---|---|---|---|---|---|---|
| 10 | 30 | 60 | 120 | 180 | 240 | |
| c-fos mRNA pPVN | route, p = 0.0027; time,p = 0.0124; route × time, p = 0.00501-1002 | |||||
| Intraperitoneal | 353.8 ± 140.91-a | 1309.1 ± 583.3 | 3522.6 ± 588.0 | 3038.2 ± 694.0 | 955.0 ± 250.0 | 1143.5 ± 0 |
| Intragastric | 767.6 ± 108.4 | 961.2 ± 787.4 | 323.5 ± 172.5 | 381.5 ± 490.3 | 70.0 ± 165.3 | 1259.4 ± 547.6 |
| c-fos mRNA mPVN | route,p = 0.3881; time, p = 0.3414; route × time,p = 0.7323 | |||||
| Intraperitoneal | 92.7 ± 33.9 | 353.9 ± 206.3 | 325.4 ± 102.0 | 499.7 ± 153.4 | 208.1 ± 41.1 | 221.7 ± 0.00 |
| Intragastric | 298.0 ± 54.6 | 306.9 ± 211.2 | 143.5 ± 83.0 | 305.1 ± 181.6 | 87.2 ± 15.8 | 239.8 ± 78.3 |
| AVP hnRNA pPVN | route, p = 0.0125; time, p = 0.0402; route × time, p = 0.6172 | |||||
| Intraperitoneal | ND1-c | 35.5 ± 27.7 | 83.9 ± 19.9 | 148.0 ± 26.0 | 161.3 ± 45.3 | 131.8 ± 10.8 |
| Intragastric | ND | 106.2 ± 23.2 | 204.9 ± 102.1 | 296.0 ± 103.7 | 394.1 ± 105.3 | 357.1 ± 113.7 |
| AVP hnRNA mPVN | route, p = 0.0003; time,p = 0.0126; route × time, p = 0.0048 | |||||
| Intraperitoneal | ND | 308.2 ± 121.3 | 422.9 ± 99.2 | 704.6 ± 167.7 | 442.4 ± 88.0 | 477.3 ± 179.7 |
| Intragastric | ND | 632.9 ± 174.8 | 948.8 ± 232.9 | 1245.7 ± 284.8 | 1819.5 ± 131.3 | 2333.7 ± 608.8 |
| CRF hnRNA pPVN | route, p = 0.0060; time,p = 0.0002; route × time, p = 0.0001 | |||||
| Intraperitoneal | 118.6 ± 62.4 | 209.4 ± 68.2 | 680.3 ± 84.0 | 61.3 ± 32.4 | 85.8 ± 43.0 | 129.2 ± 129.2 |
| Intragastric | 157.6 ± 84.5 | 77.6 ± 38.8 | 74.5 ± 42.3 | 67.0 ± 30.7 | 66.7 ± 49.3 | 123.0 ± 32.8 |
| CRF hnRNA mPVN | route, p = 0.0330; time,p = 0.0552; route × time, p = 0.1109 | |||||
| Intraperitoneal | 242.8 ± 158.2 | 97.3 ± 62.9 | 488.7 ± 117.8 | 104.4 ± 34.2 | 202.4 ± 35.8 | 356.2 ± 53.5 |
| Intragastric | 146.4 ± 82.9 | 139.3 ± 75.0 | 90.2 ± 35.9 | 58.6 ± 47.4 | 59.3 ± 59.3 | 206.3 ± 18.3 |
↵F1-a Mean ± SEM (n = 3–5 per group) levels of RNA species (as a percent of expression in the pPVN of control animals) after injection of alcohol into the peritoneum or stomach.
↵F1-1002 Statistical comparison was accomplished by two-way ANOVA with route of alcohol administration and time after injection as the variables.
↵F1-c ND, Not determined.