Biophysical properties of total barium currents in wild-type and leaner Purkinje cells
| Wild-type | tgla/+ | tgla/tgla | |
|---|---|---|---|
| Total IBa density (pA/pF) | 186.2 ± 10.0 (22) | 140.3 ± 5.2 (35)* | 61.5 ± 2.6 (51)* |
| Voltage dependence of activation | |||
| V1/2 (mV) | −22.5 ± 1.6 (8) | −21.9 ± 1.2 (14) | −24.8 ± 1.0 (18) |
| Slope factor (mV) | 4.0 ± 0.2 (8) | 4.9 ± 0.3 (14) | 6.9 ± 0.2 (18)* |
| Percent inactivation (%)1-a | 63 ± 2 (14) | 60 ± 2 (23) | 50 ± 2 (31)** |
| Voltage dependence of inactivation | |||
| V1/2 (mV) | −53.0 ± 2.9 (9) | −50.5 ± 2.1 (17) | −56.2 ± 2.7 (20) |
| Slope factor (mV) | 21.1 ± 1.8 (9) | 17.9 ± 0.6 (17) | 17.8 ± 0.7 (20) |
| Percent current reduction by 100 nM ω-AgTx IVA (%) | 82.1 ± 2.3 (3) | 79.7 ± 1.2 (10) | 49.1 ± 12.5 (7)* |
Values are means ± SEM with number of cells in parentheses.
↵F1-a Percent inactivation defined as [(Ipeak − I at 2 sec)/Ipeak].
*Significantly different from other groups; p < 0.0001.
**Significantly different from other groups; p < 0.0005.