Postnatal age at operation (d) | Operation | Age at time of study | n | Methods | |||
---|---|---|---|---|---|---|---|
B | E | M | P | ||||
5–8 | Crush | P5–P9 (+0–24 hr) | 25 | 11 | 18 | ||
6–7 | Cut | P6–P8 (+0–24 hr) | 13 | 13 | |||
6 | Cut | 2 months | 3 | 3 | 3 | ||
Controls | 2 months | 3 | 3 | ||||
7–8 | Crush | 3 months | 8 | 8 | |||
Controls | 3 months | 3 | 3 | ||||
4 | Cut | 6 months | 3 | 3 | 1 | 1 | |
Controls | 6 months | 3 | 3 | 2 | |||
7–8 | Crush | 2–3 years | 10 | 10 | 5 | 8 | 3 |
Controls | 3 years | 8 | 5 | 3 | 5 | 2 |
Methods of study: B, behavior; E, electrophysiology; M, morphology; P, pathway tracing (dextran amine injections);n, number of animals. Numbers in Methods column refer to the numbers of animals and preparations studied by the methods indicated; in some cases more than one method was applied to some preparations. A further 19 animals (P11–P27 at time of study) were used for electrophysiology studies of crush preparations (see Table 2).