Table 4.

RT-PCR primers

Subunit	Fragment size (bp)		Start	5′ Sequence 3′	End
hGABAR α-1	470	Upper	664	GGA CAA ACA GTA GAC TCT GG	684
		Lower	1111	ATT AGG GGT GTA GCT GGT TGC TGT	1134
hGABAR α-2	471	Upper	661	CTG GGC CAA TCA ATC GGA AAG GAG A	585
		Lower	1108	GAT TCG GGG CAT AAT TGG CAA CAG C

hGABAR α-3	470	Upper	734	TTT TGG GCC ATG TTG TTG GGA CAG AGA T	761
		Lower	1177	TGG TCC CCA CGA TGT TGA AGG TAG TGC T	1204

hGABAR α-4	554	Upper	928	TTG CCC AAA GTG TCC TAT GC	947
		Lower	1459	AGC CCC TAT GGT ATT AAC TGT GGT	1482

hGABAR α-5	570	Upper	86	GCT TTT CAC AGA TGC CAA CCA GTT CAG	112
		Lower	630	CCA TCT TCC GCC ACC ACC ACC GAC TTG	656

hGABAR α-6	471	Upper	634	GGA CAA ACA GTA TCT AGT GAG AC	656
		Lower	1086	GAT ATT TGG AGT CAG GAT GC	1105

hGABAR β-1	542	Upper	651	GAT GGT GTC TAA GAA GGT GGA G	672
		Lower	1170	TCA TAG GAG TAC ATG GTG GCC TTG	1193
hGABAR β-2	433	Upper	571	TAC TGG CGT GGC GAT GAT AAT G	592
		Lower	1004	GGC CCC CTC CCA AAG AAG A	986
hGABAR β-3	794	Upper	279	AGA TAA AAG GCT CGC CTA TTC TGG	302
		Lower	1050	CTT TCG CTC TTT GAA CGG TCA TTC	1073

hGABAR γ-1	465	Upper	735	CTC AAC TGA AAT CAT TCA CAC G	756
		Lower	1180	ATC TTC TTG CGG CAC AGA AAT	1200
hGABAR γ-2	558	Upper	754	GTG AAG ACA ACT TCC GG	770
		Lower	1288	CCA AGC TCC TGT TCG ACA ATC TTC	1311

hGABAR γ-3	769	Upper	56	CAG AAA GGT GGA AGA GGA TGA A	78
		Lower	802	AAA TGA CAC CCA GGA TAA AAC CAC	825
hGABA ε	314	Upper	34	CGC GGA AAT GTT GTC CAA AGT TCT	57
		Lower	328	CGG CGA TCT CAA CAG TGA CC	347

hGABAR π	591	Upper	84	CGA GGT CGG CAG AAG TGA CAA G	105
		Lower	652	TCC TGC TGC GAT CTG GTG ACT AA	674

Primers used to direct PCR amplification of human GABAR mRNAs expressed in NT2-N cells and the cDNA positive controls. The sequences of the primer pairs used to amplify each subunit, the fragment size (in base pairs), and the region amplified were included.