Primer sequences used for PCR amplification
| Gene | Product size (bp) | Primers (sense, antisense) |
|---|---|---|
| p75 | 329 | 5′-GCA CAT ACT CAG ACG AAG CCA-3′ |
| 5′-AGC AGC CAA GAT GGA GCA ATA GAC-3′ | ||
| ChAT | 377 | 5′-CTG AAT ACT GGC TGA ATG ACA TG-3′ |
| 5′-AAA TTA ATG ACA ACA TCC AAG AC-3′ | ||
| Isl-1 | 350 | 5′-GCA GCA TAG GCTTCA GCA AG-3′ |
| 5′-GTA GCA GGT CCG CAA GGT G-3′ | ||
| GAD65 | 327 | 5′-GAA TCT TTT CTC CTG GTG GTG-3′ |
| 5′-GAT CAA AAG CCC CGT ACA CAG-3′ | ||
| Calbindin28 | 276 | 5′-GCA GAA TCC CAC CTG CAG-3′ |
| 5′-GTT GCT GGC ATC GAA AGA G-3′ | ||
| Glutaminase | 560 | 5′-GCA CAG ACA TGG TTG GGA TAC TAG-3′ |
| 5′-GCA GGG CTG TTC TGG AGT CG-3′ | ||
| Smoothened | 467 | 5′-GGA CAG ACA ACC CCA AGA GC-3′ |
| 5′-CCG AGA GAG GCT GGT AAG TGG-3′ | ||
| Patched | 1297 | 5′-CAA TGG CTA CCC CTT CCT GTT CTG-3′ |
| 5′-CGG CCT CTC CTC ACA TTC CAC-3′ | ||
| glyRa2 | 330 | 5′-CAG AGT TCA GGT TCC AGG G-3′ |
| 5′-TCC ACA AAC TTC TTC TTG ATA G-3′ | ||
| GABAA-R | 589 | 5′-TGG AC(TC) CC(AT) GA(TC) AC(ACT)TT(TC) TT-3′ |
| 5′-GC(AGTC) AT(GA) AAC CA(GA)TCC ATG GC-3′ | ||
| Cyclophilin | 302 | 5′-CCA CCG TGT TCT TCG ACA TC-3′ |
| 5′-GGT CCA GCA TTT GCC ATG G-3′ |