Gal4 DNA binding hybrid | Gal4 activation hybrid | Colony color | Growth |
---|---|---|---|
PSD-95, GK (503–724) | SV 40 (amino acids 84–708) | White | − |
p53 (72–390) | MAP1A (1618–1962) | White | − |
PSD-95, GK (503–724) | MAP1A (1618–1962) | Blue | + |
PSD-95, GK (503–724) | MAP1A (1860–1962) | Blue | + |
PSD-95, GK-Cmut (503–699) | MAP1A (1860–1962) | White | − |
PSD-95, GK-Nmut (560–724) | MAP1A (1860–1962) | White | − |
p53 (72–390) | SV 40 (84–708) | Blue | + |
Yeast HF7c and Y187 cells were cotransformed with expression vectors encoding various GAL4-binding domain and GAL4 activation domain fusion proteins. Each transformation mixture was plated on two synthetic dextrose plates, one lacking tryptophan and leucine and the other lacking tryptophan, leucine, and histidine. Growth was measured on histidine-deficient plates, and color was measured by a β-galactosidase colorimetric filter assay (Fields and Song, 1989).