Effects of D2 receptor agonist and D2 receptor antagonist on D1-stimulated increase in NR1 phosphorylation in nucleus accumbens slices
| Treatment | [32P]phosphate-labeled NR1 (%) | |
|---|---|---|
| (−)Raclopride | (+)Raclopride | |
| Control | 50 ± 4 | 44 ± 19 |
| D1 agonist | 100 | 88 ± 6 |
| D1 + D2 | 49 ± 4* | 98 ± 16 |
| D2 agonist | 43 ± 7 | |
Nucleus accumbens slices were prelabeled with [32P]phosphate. Slices were incubated for a total of 15 min. Raclopride (1 μm) was added at 0 min, and D1 agonist (SKF81297, 1 μm) and D2 agonist (quinpirole, 1 μm) were added at 10 min. [32P]phosphate incorporation into NR1 was determined as described in Materials and Methods. Data were calculated as percentage of radioactivity in slices incubated with D1 agonist and represent means ± SEM (*p < 0.05, compared with D1 agonist alone, Mann-Whitney U test; n = 4–10 experiments).