Colocalization of postembedding immunoreactivity for glutamate receptor subunits GluR2/3 and GluR4 at synapses on spherical bushy cells
| Receptors (gold size) | Number of gold particles/PSDs | Average length of PSD (μm) | Average number of gold particles per PSD (range) | Number of gold particles per μm of PSD ± SE |
|---|---|---|---|---|
| Round Vesicle Synapses | ||||
| GluR2/3 (15 nm) | 170 /92 | 0.26 | 1.8 (0–6) | 7.2 ± 0.5 |
| GluR4 (5 nm) | 286 /92 | 3.1 (0–13) | 12.6 ± 1.2 | |
| Dendrite on endbulb | ||||
| GluR2/3 (15 nm) | 34 /20 | 0.24 | 1.7 (0–8) | 6.5 ± 1.9 |
| GluR4 (5 nm) | 36 /20 | 1.8 (0–5) | 7.2 ± 1.4 | |
| Number of synapses labeled with either or both antibodies | ||||
| GluR2/3 & GluR4 | GluR2/3 only | GluR4 only | No gold | |
|---|---|---|---|---|
| Round vesicle synapse | 62 (67%) | 8 (9%) | 9 (10%) | 13 (14%) |
| Dendrite on endbulb | 8 (40%) | 3 (15%) | 6 (30%) | 3 (15%) |
Thirteen cells were examined. GluR2/3 was a monoclonal mouse IgG antibody; GluR4 was the same polyclonal rabbit IgG antibody as that used in Table 2. PSD, Postsynaptic density.