Prey | Fh1 | ABP280 |
---|---|---|
Bait (LexA fusion) | ||
APP | 1.0 | 1.0 |
PS1 | 17.0 | 17.4 |
PS2 | 15.5 | 32.6 |
PS1E280A | 32.8 | 15.4 |
PS1L286V | 23.3 | 19.0 |
PS1P267S | 22.0 | 16.5 |
PS1R269H | 41.2 | 15.9 |
PS1dEx9 | 33.5 | 36.1 |
Quantitative liquid β-galactosidase assays were performed on at least three independent colonies for each combination. The numbers represent the fold activation above background. The background is defined as the amount of β-galactosidase activity detected in the yeast, with cotransformation of corresponding prey plasmids and the bait plasmid expressing APP intracellular domain–LexA fusion protein, which has the same level of β-galactosidase activity as the yeast containing the prey plasmid and the LexA bait vector plasmid without any cDNA insert.