Characterization of currents from oocytes coexpressing wild-type and EA subunits
| Voltage-dependent parameters | Activation τfast(msec) | Deactivation τ (msec) | C-type inactivation Ifinal/Ipeak | ||
|---|---|---|---|---|---|
| V1/2(mV) | k (mV) | ||||
| Wild type alone | −28.8 ± 2.3 (6) | 8.1 ± 0.9 | 2.9 ± 0.5 (5) | 11.8 ± 3.1 (21) | 0.70 ± 0.01 (10) |
| V174F | −5.3 ± 3.3 (10)* | 11.6 ± 0.9* | 5.9 ± 0.7 (4)* | 6.2 ± 0.7 (5)* | 0.68 ± 0.02 (7) |
| F184C | −13.8 ± 1.9 (8)* | 9.5 ± 0.6* | 10.4 ± 1.1 (4)* | 11.4 ± 3.0 (8) | 0.75 ± 0.01 (4)* |
| R239S | −28.0 ± 2.2 (6) | 7.8 ± 1.8 | 3.7 ± 0.5 (5) | 14.1 ± 1.9 (9) | 0.72 ± 0.02 (5) |
| F249I | −25.7 ± 2.1 (3) | 5.9 ± 0.8* | 4.2 ± 0.5 (3) | 14.2 ± 1.9 (8) | 0.67 ± 0.01 (3)* |
| E325D | −0.5 ± 2.6 (5)* | 17.4 ± 4.0* | 2.5 ± 0.2 (4) | 3.3 ± 0.5 (7)* | 0.50 ± 0.04 (8)* |
| V408A | −27.5 ± 2.2 (8) | 8.2 ± 0.6 | 2.6 ± 0.3 (8) | 3.7 ± 1.2 (5)* | 0.30 ± 0.01 (8)* |
Voltage-dependent parameters of activation (V1/2 and k) were obtained from the Boltzmann equation described in Figure 2. Time constant for activation at 20 mV (τfast) and deactivation at −50 mV (τ) were determined from exponential fits. C-type inactivation was measured at 20 mV and presented as the ratio Ifinal/Ipeak. Data are presented as mean ± SD (number of cells).
*Significance of p < 0.01 compared with wild type.