Dimensions and vesicle concentrations at reconstructed synapses
| Synapse | Stain | Dimensions | Synaptic body | Outlying vesicles | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Voxel Edge (nm) | Reconstructed volume (nm × nm × nm) | Diameter (nm) | Portion reconstructed (%) | Vesicles counted (#) | Total vesicles/SB (#) | Packing density (%) | Count (#) | Packing density (%) | ||
| 1 | Os | 3.3 | 845 × 845 × 200 | 524 | 29 | 162 | 562 | 68 | 164 | 6.5 |
| 2 | Os | 2.1 | 840 × 840 × 215 | 516 | 36 | 171 | 474 | 59 | 78 | 4.2 |
| 3 | Os | 3.0 | 900 × 900 × 205 | 362 | 34 | 89 | 265 | 60 | 67 | 4.0 |
| 4 | EPTA | 2.6 | 1470 × 1125 × 325 | 581 | 47 | 231 | 494 | 50 | 287 | 3.9 |
| 457 | 45 | 134 | 298 | 46 | ||||||
| 5 | EPTA | 2.9 | 1145 × 1145 × 380 | 477 | 75 | 139 | 186 | 26 | 208 | 3.4 |
| 6 | EPTA | 1.3 | 770 × 770 × 390 | 425 | 70 | 204 | 290 | 50 | 206 | 8.1 |
| 7 | EPTA | 1.6 | 770 × 630 × 155 | 440 | 20 | 103 | 506 | 83 | — | — |
| 8 | Os | 1.4 | 780 × 745 × 255 | 431 | 56 | 171 | 307 | 52 | 78 | 6.4 |
| 468 ± 65 | 376 ± 133 | 55 ± 16 | 5.2 ± 0.67 | |||||||
| (SD) | (SD) | (SD) | (SEM) | |||||||
Voxels were cubes having the specified length along each edge. The listed dimensions are for the backprojections used to calculate SB size and vesicle concentrations. Data for the two SBs at synapse 4 are listed. At synapses 1 and 3, a second backprojection encompassing a larger volume was computed also (data not shown).