Antibodies used to characterize molecular complexes and organelles involved in protein synthesis
| Organelles/Molecular complexes | Concentration | Reference |
|---|---|---|
| Endoplasmic reticulum | ||
| BiP (mAb) | 1:200 | StressGen Biotech, Victoria, Canada |
| Initiation factor | ||
| eIF-2β (rabbit) | 1:200 | Bommer et al., 1988 |
| Ribosomes | ||
| Ribosomal P proteins (human) | 1:500 | Elkon et al., 1985 |
| Intermediate compartment and cis-Golgi | ||
| rab1 (rabbit) | 1:100 | Saraste et al., 1995 |
| Golgi apparatus | ||
| CTR 433 (mAb) | 1:50 | Jasmin et al., 1989 |
| TGN 38 (rabbit) | 1:500 | Wilde et al., 1992 |
| Presynaptic markers | ||
| Synapsin (rabbit) | 1:1000 | Bloom et al., 1979; De Camilli et al., 1979 |
| Synaptophysin (mAb) | 1:20 | Boehringer Mannheim, Indianapolis, IN |