Extracellular solutions | Pipette solutions | |||||
---|---|---|---|---|---|---|
2 K+ | 5 K+ | 8 K+ | 10 K+ | 4.5 mm [A−] | 15 mm[A−] | |
NaCl | 156 | 153 | 150 | 148 | 3.5 | |
CsCl | 1 (5)1-a | 1 | 1 | 5 | ||
KCl | 2 | 5 | 8 | 10 | 5 | |
K-glucuronate | 140 | 130 | ||||
CaCl2 | 2 | 2 | 2 | 2 | 0.25 | 0.25 |
MgCl2 | 1 | 1 | 1 | 1 | 0.5 | |
Mg-ATP | 2 | 2 | ||||
Glucose | 15 | 15 | 15 | 15 | 10 | 10 |
HEPES | 10 | 10 | 10 | 10 | 10 | 10 |
EGTA | 5 | 5 | ||||
QX 314-Br− | 4 | 5 |
↵F1-a CsCl (5 mm) and K+ (10 mm) were used only to recordIGABA but not to record sIPSCs to avoid too strong depolarization of network neurons.