IRAP and NMMA blockade of gp41-induced NO production
| Treatment | Medium | IRAP | Anti-IL-1β | NMMA |
|---|---|---|---|---|
| Control | 0.9 ± 0.1 | 0.7 ± 0.2 | 0.45 ± 0.12 | 1.1 ± 0.1 |
| gp41 | 3.9 ± 0.2* | 0.9 ± 0.1** | 0.72 ± 0.31** | 0.8 ± 0.1** |
| IFN-γ | 0.9 ± 0.1 | 0.9 ± 0.1 | 0.74 ± 0.18 | 0.9 ± 0.2 |
| gp41 + IFN-γ | 22.0 ± 3.2* | 0.9 ± 0.1** | 1.14 ± 0.23** | 2.9 ± 0.9** |
Highly enriched astrocyte cultures were incubated for 5 d with supernatants derived from microglial cell cultures treated with medium alone (Control) or medium containing gp41 (20 μm), IFN-γ (200 U/ml), gp41 plus IFN-γ in the absence or presence of IRAP (200 ng/ml), anti-IL-1β antibody (10 μg/ml), or NMMA (500 μm) for 48 h. Data are mean ± SEM of triplicates and are representative of three separate experiments.
*p < 0.05 versus corresponding control in each column.
**p < 0.05 versus medium group in each row.