Interaction of NIP with Npn-1 determined by filter assay of β-galactosidase activities and His+ selection
| Npn-1/C | Npn-1/C (ΔSEA) | Vector | ||||
|---|---|---|---|---|---|---|
| β-galactosidase activity | His+ | β-galactosidase activity | His+ | β-galactosidase activity | His+ | |
| NIP | +++++ | + | − | − | − | − |
| NIP1–148 | − | − | ||||
| NIP120–333 | ++++ | + | − | − | − | − |
| NIP120–248 | +++ | + | ||||
| NIP221–333 | + | + | ||||
| Vector | − | − | ||||
Npn-1/C, The cytoplasmic domain of Npn-1; Npn-1/C (ΔSEA), the cytoplasmic domain of Npn-1 with the deletion of the last three amino acids; +, positive reaction; −, negative reaction.