Behavior of wild-type C. elegans (N2), reserpine-treated N2, and two cat-1 mutants
| Behavior | N2 | N2 + reserpine | cat-1(e1111) | cat-1(n733) |
|---|---|---|---|---|
| Movement on plates (bends/min)2-a | ||||
| On food | 11.0 ± 1.1 | 15.7 ± 1.8* | 17.7 ± 1.6* | 15.6 ± 1.9* |
| Off food | 18.5 ± 2.1 | 17.9 ± 2.4 | 18.7 ± 2.6 | 19.0 ± 1.9 |
| Thrashing in liquid (bends/min)2-b | 217 ± 19 | ND | 216 ± 7 | 214 ± 12 |
| Egg laying (No. of eggs in utero)2-c | ||||
| 14°C | 14.8 ± 2.2 | 13.1 ± 1.6 | 15.5 ± 2.5 | 15.1 ± 0.6 |
| 20°C | 15.9 ± 3.2 | 25.1 ± 3.8** | 27.3 ± 2.2** | 23.8 ± 1.5** |
| Male mating2-d | ||||
| % No progeny | 30 (17/56) | ND | 90 (46/51)*** | 100 (58/58)*** |
| % 1–100 progeny | 13 (7/56) | 8 (4/51) | 0 (0/58) | |
| % >100 progeny | 57 (32/56) | 2 (1/51) | 0 (0/58) |
ND, Not determined.
↵F2-a Movement on plates was determined for 10 groups of 5 individuals either on food or off food for each genotype, as described in Materials and Methods. Means ± SEMs are given.
↵F2-b Thrashing in liquid was determined for 10 groups of 10 individuals as described in Materials and Methods. Means of the 10 groups ± SEMs are given.
↵F2-c The number of fertilized eggs in utero was counted in groups of 19–63 adult hermaphrodites raised at particular temperatures. Means of four groups ± SEMs are given.
↵F2-d Single males were placed with single “females” as described in Materials and Methods. The percentages of males that produced a given number of progeny are listed with numbers of individuals in parentheses. Mating was also evaluated forcat-1 mutants carrying the amber suppressor sup-5(Waterston and Brenner, 1978). Male progeny of a cross betweensup-5/+ males and cat-1 hermaphrodites (approximately half sup-5/+;cat-1 and half +/+;cat-1) were tested for mating ability. With thecat-1(e1111) amber mutation, there was significantsup-5-dependent rescue of mating: 47% no progeny, 22% 1–100 progeny; 31% > 100 progeny (n = 58; different from cat-1(e1111) alone with p < 0.01, χ2 test). With the cat-1(n733) missense mutation, there was no sup-5-dependent rescue of mating: 0% mated (n = 20).
*cat-1(e1111), cat-1(n733), and reserpine-treated N2 were significantly different from N2; p < 0.01 (Student’s t test).
**cat-1(e1111), cat-1(n733), and reserpine-treated nematodes were all significantly different from N2 at 20°C; p < 0.01 (Student’s t test).
***cat-1(e1111) and cat-1(n733) were significantly different from N2; p < 0.01 (χ2 test).