Effects of EGTA on measured domain size (FWHM) and the magnitude (peak ΔF/F) of the OGB-5N fluorescence transients
| [EGTA] | 10/50 μm | 500 μm | 2 mm |
|---|---|---|---|
| FWHM (μm) | |||
| mean ± SD | *1.54 ± 0.69 | *1.69 ± 0.58 | *1.51 ± 0.52 |
| n | 9 | 3 | 9 |
| Peak ΔF/F | |||
| mean ± SD | **0.14 ± 0.06 | **0.18 ± 0.13 | **0.12 ± 0.05 |
| n | 16 | 7 | 17 |
Peak ΔF/F is the averaged maximum ΔF/F of transients measured at a single spot location or the averaged maximum ΔF/F of the three or four largest transients from a scan. Only traces exhibiting a time-to-peak of <2 msec and a rapid decay time constant were used for further analysis of peak ΔF/F. *p > 0.7; **p > 0.2; Student’st test.