Two-hybrid analysis of 4.1N
| Library, rat cortex and hippocampus | No. transformants (2 × 106) | No. His+(28) | No. His+ and β-gal positive (13) |
|---|---|---|---|
| Binding domain fusion protein (pPC97) | Activation domain fusion protein (pPC86) | Histidine prototrophy | β-Gal filter assay |
| 4.1N (aa 679–879) | NuMA (aa 1440–1913) | + | + |
| NuMA (aa 1440–1913) | 4.1N (aa 679–879) | + | + |
| 4.1N (aa 1–321) | NuMA (aa 1440–1913) | − | − |
| NuMA (aa 1440–1913) | 4.1N (aa 1–321) | − | − |