Table 4.

Saline and ethanol (difference score) locomotor responses in male B6D2 F₂ intercross animals (N = 900)

Treatment	Trial/analysis	0–5 min	5–10 min	10–15 min	15–20 min	5–20 min
Saline	1	2052 ± 930	1772 ± 810	1645 ± 750	1602 ± 827	5017 ± 2141
	2	2095 ± 1013	1855 ± 931	1718 ± 876	1655 ± 910	5212 ± 2487
	Average^4-a	2068 ± 854	1809 ± 749	1679 ± 695	1626 ± 738	5115 ± 2033
	Average-C	2009 ± 823				4985 ± 1968
	F test	1.3	1.36	1.38	1.39	1.3
	Test–retest	0.54	0.47	0.45	0.44	0.54
	Test–retest-C	0.71				0.71
Ethanol	1	970 ± 1460	410 ± 1610	48 ± 1430	−4 ± 1340	450 ± 4050
	2	1180 ± 1490	910 ± 1580	480 ± 1430	370 ± 1330	1760 ± 3990
	Average^4-b	1080 ± 1120	660 ± 1240	270 ± 1140	180 ± 1030	1110 ± 3170
	Average-C	1090 ± 1100				1090 ± 3160
	F test	1.73	1.65	1.57	1.68	1.6
	Test/retest	0.15	0.21	0.22	0.22	0.25*
	Test/retest-C	0.38				0.51*

The saline and ethanol responses were determined as described in the legends to Tables 1 and 2. The saline–ethanol challenge was repeated in all animals 7–10 d after the first trial. For each interval data are also presented for the average of trial₁and trial₂. The F test compared the average of the variances from trials 1 and 2 to the variance associated with the average activity. Test–retest data were calculated as the correlation between trial₁ and trial₂. The absolute value of the difference between trial₁ and trial₂ was calculated and used to rank order the animals. The 10% of the animals showing the poorest test–retest reliability were censored from the data set. The censored (C) values for the average response and the test–retest reliability are also presented (0–5 and 5–20 min intervals, only). All data are presented as the mean ± SD.
*Significantly different from the 0–5 min value; p < 0.01.
↵F4-a Correlation matrix for the average saline response: Time (min) 0–5 5–10 10–15 15–20 0–5 1.00 0.78 0.71 0.63 5–10 1.00 0.84 0.75 10–15 1.00 0.81 15–20 1.00.
↵F4-b Correlation matrix for average ethanol response: Time (min) 0–5 5–10 10–15 15–20 0–5 1.00 0.63 0.50 0.41 5–20 1.00 0.840.73 10–15 1.00 0.81 15–20 1.00.
The values in bold are significantly different (p < 0.01) from the relative value in the 0–5 min column.