Comparison of macroscopic current properties of mutant andwtGluR6
| τDa(msec) | Rise timeb(msec) | Vrev1-c(mV) | G+80/G−801-d | |
|---|---|---|---|---|
| wt GluR6 | 6.0 ± 0.2 (54) | 1.9 ± 0.06 (35) | 1.3 ± 1.3 (9) | 1.5 ± 0.2 (14) |
| GluR6(S684C) | 6.1 ± 0.1 (51) | 2.0 ± 0.05 (29) | 1.2 ± 1.4 (10) | 1.8 ± 0.3 (10) |
All measurements were made for current responses to rapid application of 1 mm glutamate for 100 msec.
aDesensitization time course was fit to a single exponential using CLAMPFIT software.
bMeasured as time from 10 to 90% of peak current response.
↵F1-c Holding potential was stepped from −60 mV to potentials ranging from −100 to +100 mV in 20 mV increments every 20 sec before application of glutamate; peak current responses were measured.
↵F1-d Ratio of conductance measured at +80 mV to that at −80 mV.