X11α (region 373–436) is the minimal binding site for mLin-2
| X11α peptides | mLin-2 | Munc-18-1 |
|---|---|---|
| 163–436 | +++ | +++ |
| 163–315 | − | +++ |
| 315–436 | +++ | − |
| 315–373 | − | − |
| 373–436 | ++ | − |
| 373–392 | − | NT |
| 373–402 | − | NT |
| 390–408 | − | NT |
| 390–425 | − | NT |
X11α peptides fused to the GST protein were used to pull down mLin-2 from a 293 cells lysate or mouse brain extract. Bound protein was detected by Western blot or overlay assays. Binding to X11α (region 373–436) was reproducibly weaker than to larger peptides. NT, Non-tested.