Quantification of colocalization of sec6, synapsin1, and FM1-43 fluorescence in stimulated synapses
| sec6 | synapsin1 | FM1-43 | % of sec6 sites |
|---|---|---|---|
| + | − | − | 66 ± 9 |
| + | + | − | 14 ± 9 |
| + | + | + | 16 ± 6 |
| + | − | + | 4 ± 5 |
sec6 sites were counted and scored for the presence or absence of synapsin1 and/or FM1-43. Percentages were obtained by dividing the number of each group by the total number of sec6 sites.