Table 1.

Composition of mammalian and earthworm salines

Solution ^#	Designated name	Composition (in mm)
1	Ca²⁺-free hypotonic PBS	77 NaCl, 2.6 KCl, 4.0 Na₂HPO₄, and 1.0 KH₂PO₄
2	Physiological mammalian saline	133 NaCl, 2.6 KCl, 2.0 CaCl₂, and 10.0 HEPES, pH 7.4
3	Ca²⁺-free slightly hypotonic mammalian saline	133 NaCl, 2.6 KCl, 10.0 HEPES, and 0.5–2.0 EGTA, pH 7.4
4	Ca²⁺-free hypotonic mammalian saline	66.5 NaCl, 2.6 KCl, 10.0 HEPES, and 0.5–2.0 EGTA, pH 7.4
5	Physiological Krebs’ solution	124 NaCl, 2.0 KCl, 26 NaHCO₃, 1.3 MgSO₄, 1.2 KH₂PO₄, 1.2 CaCl₂, 10.0 dextrose, and 6.0 Na ascorbate, pH 7.4
6	Ca²⁺-free (isotonic) Krebs’ solution	124 NaCl, 2.0 KCl, 26 NaHCO₃, 2.5 MgSO₄, 1.2 KH₂PO₄, 10.0 dextrose, 6.0 Na ascorbate, and 0.5–2.0 EGTA, pH 7.4
7	Physiological earthworm saline	40 NaCl, 10 Na₂SO₄, 0.5 K₂SO₄, 0.5 MgCl₂, 3.0 CaCl₂ 1.3 TRIS, and 1.5 HEPES, pH 7.4
8	Ca²⁺-free earthworm saline	40 NaCl, 10 Na₂SO₄, 0.5 K₂SO₄, 0.5 MgCl₂, 1.3 TRIS, 1.5 HEPES, and 0.5–1.0 EGTA, pH 7.4