Apolipoprotein E nuclear localization assay
| Experiment | ApoE particle | Intracellular E3 (ng/mg cell protein) | Intracellular E3 + NLS (ng/mg cell protein) | ApoE cleared from medium | Percent cleared | Nuclear apoE |
|---|---|---|---|---|---|---|
| A | Discs 10 μg/ml | 179.5 ± 2.2 | 143.3 ± 15.9 | 4.1 μg | 41% | ND |
| B | Discs 10 μg/ml | 111.2 ± 17 | 137.8 ± 9.1 | 4.3 μg | 43% | ND |
| C | Discs 25 μg/ml | 692.8 ± 147 | 784.1 ± 337 | — | — | ND |
| D | Discs 10 μg/ml plus lipid poor apoE 10 μg/ml | 147.5 ± 6.7 | 151.7 ± 12.5 | 12.3 μg | 62% | ND |
| E | ApoE βVLDL 10 μg/ml | 52.8 ± 7.0 | 17.9 ± 2.2 | — | — | ND |
| F | Mouse βVLDL plus lipid poor apoE 10 μg/ml | 52.3 ± 0.2 | 21.9 ± 2.3 | 8.2 μg | 82% | ND |
ND, Not detected.