Oligonucleotide primers used for RT-PCR amplification of Erg1, Erg2 and Erg3 sequences
| Primers | Source (GenBank accession number) | nt position | Sequence (5′ to 3′) | Predicted length amplified product |
|---|---|---|---|---|
| Erg1-1s | U04270 | (1298–1323) | gaaggtcacccaggtcctgtccctg | 805 bp |
| Erg1-1r | (2078–2103) | gaagatcttctctgagttggtgttg | ||
| Erg2-2s | AF016192 | (237–260) | tcacgggcccgaacactccaagca | 673 bp |
| Erg2-2r | (888–910) | acaggtaccgcgctgtgattcgtc | ||
| Erg3-3s | AF016191 | (825–848) | atacgaaggccttgatacagccta | 316 bp |
| Erg3-3r | (1117–1141) | tctgatgtggatcccaacaggctt | ||
| Erg1-4s | Z96106 | (1994–2013) | tccagcggctgtactcgggc | 597 bp |
| Erg1-4r | (1569–2591) | tagaccaaaagtggtctgagaactc | ||
| Erg2-5s | AF016192 | (2356–2376) | aggacctggattgctggcatc | 400 bp |
| Erg2-5r | (2735–2756) | aggtgaggctagaggtgacagg | ||
| Erg3-6s | AF016191 | (3216–3236) | cagagagctgcctggggcatc | 428 bp |
| Erg3-6r | (3624–3644) | gtcaagctctgacgatgcatc |