CDR3 sequences and germline VH gene segments of anti-HS scFv antibodies
| Clone | CDR3 sequence | VH family | DP segment |
|---|---|---|---|
| AO4B05 | LKQQGIS | 3 | 53 |
| AO4B08 | SLRMNGWRAHQ | 3 | 47 |
| AO4F12 | AMTQKKPRKLSL | 1 | 7 |
| RB4CB9 | HAPLRNTRTNT | 3 | 38 |
| RB4CD12 | GMRPRL | 3 | 38 |
| RB4EA12 | RRYALDY | 3 | 32 |
| RB4EG12 | SGRKYFRARDMN | 1 | 4 |
Antibodies with the prefix AO were obtained after panning against GAGs from mouse skeletal muscle, whereas antibodies with the prefix RB were selected by panning against GAGs isolated from human skeletal muscle. CDR3 sequences are shown in single-letter amino acid code. VH families and DP segments were deduced from the Sanger Centre Germline Quiry (http://www.sanger.ac.uk/DataSearch/gq_search.shtml) by applying the full-length VH sequences of the anti-HS scFv antibody clones (nomenclature according to Tomlinson et al., 1992).