Injection sites | rAAV injection | 6-OHDA injection 7 μg free base in 2 μl per site | |||||||
---|---|---|---|---|---|---|---|---|---|
Striatum | Nigra | ||||||||
1 | 2 | 3 | 1 | 2 | 1 | 2 | 3 | 4 | |
AP | +1.2 | +0.2 | −0.8 | −4.8 | −5.8 | +1.3 | +0.4 | −0.4 | −1.3 |
ML | −3.0 | −3.4 | −4.4 | −2.0 | −2.0 | −2.6 | −3.0 | −4.2 | −4.5 |
DV | −5.51-a | −5.51-a | −5.51-a | −7.2 | −7.2 | −5.0 | −5.0 | −5.0 | −5.0 |
Tooth bar | 0.0 | 0.0 | 0.0 | −2.3 | −2.3 | 0.0 | 0.0 | 0.0 | 0.0 |
AP and ML are mm from bregma; DV is mm from dura.
↵F1-a Intrastriatal injections start at DV −5.5 from the dura, and the micropipette is pulled up to −4.5 and −3.5 during injection.