Table 1.

Stereotaxic coordinates for the rAAV and 6-OHDA injections

Injection sitesrAAV injection6-OHDA injection 7 μg free base in 2 μl per site
StriatumNigra
123121234
AP+1.2+0.2−0.8−4.8−5.8+1.3+0.4−0.4−1.3
ML−3.0−3.4−4.4−2.0−2.0−2.6−3.0−4.2−4.5
DV−5.51-a −5.51-a −5.51-a −7.2−7.2−5.0−5.0−5.0−5.0
Tooth bar0.00.00.0−2.3−2.30.00.00.00.0
  • AP and ML are mm from bregma; DV is mm from dura.

  • F1-a Intrastriatal injections start at DV −5.5 from the dura, and the micropipette is pulled up to −4.5 and −3.5 during injection.