Quantitative RT-PCR analysis
| Genotype | PLP | MBP | MAG | MOG | OMgp | CGT | N-CAM140 | N-CAM120 |
|---|---|---|---|---|---|---|---|---|
| wt | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| plp−/− | − | 1.2 ± 0.2 | 1.1 ± 0.4 | 0.8 ± 0.5 | 1.1 ± 0.3 | 1.3 ± 0.4 | 1.3 ± 0.2 | 1.1 ± 0.4 |
| mbp−/− | 1.4 ± 0.5 | − | 3.4 ± 0.7 | 1.3 ± 0.4 | 0.9 ± 0.2 | 1.1 ± 0.2 | 1.4 ± 0.4 | 1.1 ± 0.1 |
| mag−/− | 1.3 ± 0.4 | 1.5 ± 0.3 | − | 0.8 ± 0.2 | 0.9 ± 0.5 | 0.8 ± 0.5 | 1.6 ± 0.7 | 0.7 ± 0.3 |
| plp−/−mag−/− | − | 0.9 ± 0.4 | − | 1 ± 0.4 | 1.1 ± 0.2 | 0.8 ± 0.4 | 0.8 ± 0.5 | 1.2 ± 0.3 |
| plp−/−mbp−/− | − | − | 2.7 ± 0.4 | 1.4 ± 0.3 | 1.2 ± 0.4 | 1.2 ± 0.5 | 1.3 ± 0.5 | 1.2 ± 0.5 |
| plp−/−mbp−/−mag−/− | − | − | − | 0.9 ± 0.4 | 0.7 ± 0.3 | 1.1 ± 0.3 | 0.7 ± 0.3 | 1.5 ± 0.2 |
32P-dCTP-labeled fragments were separated by PAGE, analyzed with a PhosphorImager (mean ± SEM), and normalized to the wt-expression of GAPDH and cyclophilin. The level of significance of MAG expression in mbp−/− and in plp−/−mbp−/− mutants (boldface) was determined by Student's t test (p < 0.08 and p < 0.06, respectively).