Degenerate primers used for PCR amplification of protein tyrosine kinases
| Primer | Specificity | Direction | Sequence |
|---|---|---|---|
| HRDLAARN | PTKs | Sense | CCGGATCCA(Y)(M)GIGA(Y)(Y)GCICI(M)GIAA |
| D(V/I)WS(Y/F)G(V/I) | PTKs | Anti-sense | CCGAATTCA(Y)ICC(R)(W)AIGACCAIAC(R)TC |
| QIAKGM | Erbs | Sense | GCGGATCCA(R)AT(W)GCIAA(R)GGIATG |
| TIDVYM | Erbs | Sense | GCGAATTCAT(R)TAIAC(R)TC(D)ATIGT |
| MVKCWM | Erbs | Anti-sense | CATCCA(R)CA(Y)TTIACCAT |
Primer names are given in single letter amino acid notation. Degeneracies in the primer sequences are indicated by single letters: Y = C or T; M = A or C; R = A or G; W = T or A; H = A, C or T; D = A, G, or T; I = inosine. The oligonucleotides corresponding to the 3′ primers were synthesized in the anti-sense direction. The underlined sequences at the 5′ end of the oligonucleotides contain restriction endonuclease sites to facilitate cloning.