RT-PCR gene-specific primers
| Oligo | Specificity | Sequence | Position | Ta(°C) | Predicted product size (bp) |
|---|---|---|---|---|---|
| Trp1-1 | Rat Trp1 | 5′-CGTAAGCCCACCTGTAAGAAGATAA-3′ | 1003–10271-a | 53.5 | 373 |
| Trp1-2 | Rat Trp1 | 5′-CCAAGTAAAGGGAATTCATAACAAAG-3′ | 1350–13751-a | ||
| Trp2-1 | Rat Trp2 | 5′-AGCCAGCGGTAGTGCGTCGTCT-3′ | 369–3901-b | 60.8 | 413 |
| Trp2-2 | Rat Trp2 | 5′-CTTAAACTCAGGCTCCTTCCGTGCA-3′ | 757–7811-b | ||
| Trp3-1 | Rat Trp3 | 5′-TGTAACTATGGTGGTCGTTCTGCTCA-3′ | 45–701-c | 55.2 | 363 |
| Trp3-2 | Mouse Trp3 | 5′-TGATATCGTGTTGGCTGATTGAGAAT-3′ | 2266–22911-d | ||
| Trp4-1 | Rat Trp4 | 5′-CCTACTTGAATGCCGTGGAAAAG-3′ | 220–2421-e | 56.3 | 343 |
| Trp4-2 | Rat Trp4 | 5′-AAGATGATAGGCGTGATGTCTGG-3′ | 540–5621-e | ||
| Trp5-1 | Rat Trp5 | 5′-AGTTTGTGGGAGCTACTATGTTTGG-3′ | 23–471-f | 54.9 | 431 |
| Trp5-2 | Mouse Trp5 | 5′-TTCTTCTGTTAGCCCCTCATTTGTT-3′ | 2601–26251-g | ||
| Trp6-1 | Rat Trp6 | 5′-TCTTTCTGAAGTGAAGTCGGTGGTCA-3′ | 33–581-h | 55.1 | 465 |
| Trp6-2 | Mouse Trp6 | 5′-CTCCTTGTACTTGATTGTTTGTTGTGTGC-3′ | 2344–23721-i | ||
| Trp7-1 | Mouse Trp7 | 5′-GCAAAGTACAACCCAGCGTTTACCAC-3′ | 1913–19381-j | 57.2 | 558 |
| Trp7-2 | Mouse Trp7 | 5′-CACGTATCTCTTTATGAGCCGCTTCA-3′ | 2445–24701-j |
a–jPrimer positions are given as the nucleotide sequences for the available rat or mouse transient receptor potential protein (Trp) cDNA with the following GenBank accession numbers:
↵F1-a AF061266,rattus norvegicus Trp1 mRNA;
↵F1-b AF136401, rattus norvegicus Trp2 mRNA;
↵F1-c AF061875 rattus norvegicusTrp3 mRNA;
↵F1-d AF190645 mus musculusTrp3 mRNA;
↵F1-e AB008889, rattus norvegicus Trp4 mRNA;
↵F1-f AF061876,rattus norvegicus Trp5 mRNA;
↵F1-g AF060107, mus musculus Trp5 mRNA;
↵F1-h AF061877, rattus norvegicus Trp6 mRNA;
↵F1-i AF057748m mus musculus Trp6;
↵F1-j AF139923, mus musculus Trp7 mRNA.