Comparison of the effect of H2O2 on the rotenone-induced NAD(P)H signal in the presence or absence of glucose
| ΔNAD(P)H nmol/mg protein | ||||
|---|---|---|---|---|
| Control | 25 μmH2O2 | 100 μmH2O2 | 500 μmH2O2 | |
| + Glucose | 1.054 ± 0.14 | 0.840 ± 0.13* | 0.544 ± 0.08* | 0.518 ± 0.07* |
| − Glucose | 1.285 ± 0.17 | 0.850 ± 0.17* | 0.567 ± 0.09* | 0.418 ± 0.06* |
Synaptosomes were incubated with H2O2 for 5 min, then rotenone (2 μm) was added, and the increase in the NAD(P)H signal [ΔNAD(P)H] was measured as shown in Figure 3,inset. Data are mean ± SEM of three independent experiments. Values obtained in samples containing H2O2 are significantly different (
↵* ) from the corresponding controls. Differences between values obtained at a given H2O2 concentration in glucose-containing or glucose-free medium are not statistically different.