ChAT activities of all regions after 7 weeks of CytC or NGF treatment
| Group | Frontal | Parietal | Occipital | Cingulate | Hippocampus | Olfactory bulbs | % across all regions |
|---|---|---|---|---|---|---|---|
| PBS/CytC | 44.3 ± 1.2 (100) | 35.5 ± 1.2 (100) | 42.5 ± 1.2 (100) | 50.6 ± 2.3 (100) | 46.0 ± 1.3 (100) | 26.3 ± 0.8 (100) | 100 ± 1.4 |
| PBS/NGF | 69.5 ± 3.4 (157)* | 44.1 ± 1.8 (124)* | 64.3 ± 4.0 (151)* | 75.0 ± 5.6 (148)* | 58.0 ± 1.5 (126)* | 37.9 ± 1.9 (144)* | 141.6 ± 6.3* |
| Low-192Sap/CytC | 30.2 ± 2.4 (68)** | 17.3 ± 2.3 (49)** | 12.6 ± 1.1 (30)** | 17.9 ± 3.1 (35)** | 25.1 ± 3.4 (55)** | 15.1 ± 0.7 (57)** | 49.0 ± 4.5** |
| Low-192Sap/NGF | 46.7 ± 4.2 (105)* | 23.3 ± 3.1 (66)*** | 13.7 ± 2.0 (32) | 22.9 ± 7.3 (45) | 30.3 ± 6.1 (66) | 22.2 ± 4.2 (86)* | 66.4 ± 10.0* |
| High-192Sap/CytC | 15.5 ± 3.1 (35)** | 8.8 ± 3.0 (25)** | 7.5 ± 1.1 (18)** | 7.6 ± 1.9 (15)** | 7.1 ± 3.4 (15)** | 2.3 ± 0.9 (9)** | 19.3 ± 5.4** |
| High-192Sap/NGF | 21.8 ± 3.0 (49) | 8.7 ± 1.8 (24) | 7.7 ± 0.6 (18) | 6.5 ± 0.3 (13) | 10.7 ± 2.8 (23) | 4.2 ± 1.6 (16) | 23.9 ± 3.6 |
ChAT activity was measured in homogenates from bilateral dissections by incorporation of [14C]acetyl CoA into [14C]acetylcholine. Means of the combined bilateral dissections (nanomoles of ACh per hour per milligram of protein) ± SEM are shown, and percentages, given in parentheses, are based on the comparison with the PBS/CytC only. Treatment effects were obtained by comparing corresponding groups only (PBS/CytC vs PBS/NGF, low-192Sap/CytC vs low-192Sap/NGF and high-192Sap/CytC vs high-192Sap/NGF). Lesion effects were determined by comparing PBS/CytC with either low-192Sap/CytC or high-192Sap/CytC. Frontal cortex: *NGF effect, corresponding CytC group, p < 0.0001 and 0.0005; **192Sap effect, PBS/CytC p < 0.005 and 0.0001. Parietal cortex: *NGF effect, corresponding CytC group,p < 0.05; ***p = 0.065; **192Sap effect, PBS/CytC, p < 0.0001. Occipital cortex: *NGF effect, corresponding CytC group, p < 0.0001; **192Sap effect, PBS/CytC, p < 0.0001. Cingulate cortex: *NGF effect, corresponding CytC group, p < 0.001; **192Sap effect, PBS/CytC, p < 0.0001. Hippocampus: *NGF effect, corresponding CytC group, p < 0.05; **192Sap effect, PBS/CytC, p < 0.0005 and 0.0001. Olfactory bulbs: *NGF effect, corresponding CytC group, p < 0.005 and 0.05; **192Sap effect, PBS/CytC, p < 0.005 and 0.0001. Across all regions: *NGF effect, corresponding CytC group,p < 0.0001 and 0.05; **192Sap effect, PBS/CytC,p < 0.0001.