Maximal Ca2+ responses (in μm) of spinal cord astrocytes to P2 agonists
| [Agonist] (m) | ATP | UTP | MeS | ADPβS | ATP-suramin | ATP-PPADS | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | KO | WT | KO | WT | KO | WT | KO | WT | KO | WT | KO | |
| 10−8 | _ | 0.0 | _ | 0.0 | _ | _ | 0.0 | _ | ||||
| 3 × 10−8 | _ | 0.01 | _ | 0.02 | _ | 0.02 | _ | |||||
| 10−7 | _ | 0.07 | _ | 0.3 | 0.2 | 0.05 | 0.03 | _ | ||||
| 3 × 10−7 | 0.0 | 0.4 | _ | 0.4 | 0.4 | _ | 0.02 | _ | ||||
| 10−6 | _ | 1.5 | 0.08 | 0.4 | _ | _ | 0.09 | 0.01 | 0.0 | 0.03 | 0.04 | _ |
| 3 × 10−6 | 1.4 | 1.7 | _ | 1.2 | 0.9 | _ | _ | _ | 0.04 | _ | 0.08 | _ |
| 10−5 | 3.2 | _ | _ | 0.9 | 0.06 | 0.4 | 0.1 | 0.3 | 0.09 | 0.3 | _ | |
| 3 × 10−5 | _ | _ | 0.7 | _ | _ | 0.9 | 0.2 | 0.5 | _ | 0.4 | _ | |
| 10−4 | 4.1 | 1.9 | 1.4 | _ | 0.2 | 1.0 | 0.3 | 0.4 | 0.2 | 0.4 | 0.05 | |
| 3 × 10−4 | _ | _ | 1.5 | _ | 0.5 | _ | 0.9 | 0.4 | 0.2 | _ | 0.09 | |
| 10−3 | _ | _ | _ | _ | 1.6 | 1.7 | 1.2 | _ | 0.3 | _ | 0.1 | |
Changes in intracellular calcium basal levels (μm) induced by exposing fura-2 AM-loaded spinal cord astrocytes from WT and Cx43 KO animals to purinergic agonists (ATP, UTP, 2-Me-S-ATP, ADP-β-S) and to ATP in the presence of 100 μm suramin and PPADS. The values displayed in the table were obtained from 60–90 cells of two to three independent experiments.